Levels and/or sustainability of DNA-based gene expression

US8716462B2 · kind B2 · utility

Assignees

• Carnegie Institution of Washington · US
• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Andrew Fire · Stanford, US
• Lia Gracey · Stanford, US
• Weng-Onn Lui · Stockholm, SE

Key dates

• Filing date: Jan 29, 2007
• Grant date: May 6, 2014
• Expiry date: Mar 14, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/18
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention encompasses methods for improving the level and/or sustainability of expression for a target nucleic acid in a eukaryotic cell comprising: (a) modifying the target nucleic acid to introduce or to comprise signals that limit or constrain the positions of nucleosome cores, and (b) introducing the modified target nucleic acid into the eukaryotic cell, wherein the modified target nucleic acid has improved levels and/or sustainability of expression compared to original unmodified nucleic acid.