Modulation of ABCG2-mediated urate transport to treat hyperuricemia and gout

US8722338B2 · kind B2 · utility

Assignee

• THE JOHNS HOPKINS UNIVERSITY · US

Inventors

• Michael Kottgen · Glückstadt, DE
• Josef Coresh · Pikesville, US
• William Guggino · Baltimore, US
• Anna Kottgen · Glückstadt, DE
• Owen Woodward · Columbia, US

Key dates

• Filing date: Mar 11, 2010
• Grant date: May 13, 2014
• Expiry date: Oct 9, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Genome-wide association studies (GWAS) was recently used to identify SNPs in a genomic region on chromosome 4 that associate with serum urate levels and gout. The present disclosure shows that human ATP-binding cassette, subfamily G, 2 (ABCG2), encoded by the ABCG2 gene contained in this region, is a hitherto unknown urate efflux transporter. The present disclosure further shows that native ABCG2 is located in the brush border membrane of kidney proximal tubule cells, where it mediates renal urate secretion. Introduction of the mutation Q141K encoded by the common SNP rs2231142 by site-directed mutagenesis resulted in reduced urate transport rates compared to wild-type ABCG2. Data from a population-based study of 14,783 individuals support rs2231142 as the causal variant in the region and show highly significant associations with urate levels and gout.