Method and composition for a protein transduction technology and its applications
US8722348B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 28, 2008 |
| Grant date | May 13, 2014 |
| Priority date | — |
| Expiry date | Apr 27, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/15
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery, a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.