Method for preparing a counter-tagged population of nucleic acid molecules
US8722368B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 29, 2013 |
| Grant date | May 13, 2014 |
| Priority date | — |
| Expiry date | Mar 29, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B20/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.