Methods of making di-tagged DNA libraries from DNA or RNA using double-tagged oligonucleotides
US8722585B2 · kind B2 · utility
57Cited by
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45Claims
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Key dates
| Filing date | Jun 7, 2012 |
| Grant date | May 13, 2014 |
| Priority date | — |
| Expiry date | Jun 7, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are methods, compositions and kits related to making double-tagged DNA libraries from RNA/DNA samples. A double-tagged oligonucleotide (DTO) is employed to efficiently add two different tags to ends of DNAs to make a double-tagged DNA libraries. Also disclosed are methods to make mate pair libraries using the double-tagged oligonucleotide, and methods to make double-tagged single stranded DNA. The double-tagged DNA libraries of the invention are ready to be used on next generation sequencing machines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.