System and method for automatically determining optimal excitation and emission wavelengths of a fluorophore
US8723139B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 9, 2011 |
| Grant date | May 13, 2014 |
| Priority date | — |
| Expiry date | Jun 13, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6421
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system for performing spectrofluorometry of a fluorophore sample is provided. The system includes an input module that receives user input corresponding to spectrofluorometer settings. A control module transmits control signals for controlling the spectrofluorometer during respective wavelength scans of a fluorophore sample and a blank sample. The control signals provide for automatic execution of the wavelength scans over an excitation wavelength range and an emission wavelength range. A signal-to-background determination module automatically determines multiple signal-to-background ratios based on fluorescence measurements of the fluorophore sample and the blank sample received from the spectrofluorometer. A signal-to-background analysis module automatically determines the maximum signal-to-background ratio from the multiple signal-to-background ratios.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.