Reverse cumate repressor mutant
US8728759B2 · kind B2 · utility
3Cited by
14References
37Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 3, 2005 |
| Grant date | May 20, 2014 |
| Priority date | — |
| Expiry date | Jul 10, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/21
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of CymR variants with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variants, fusion proteins incorporating such variants, and their use in the control and expression of polynucleotides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.