Closed-system multi-stage nucleic acid amplification reactions
US8728765B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 15, 2012 |
| Grant date | May 20, 2014 |
| Priority date | — |
| Expiry date | Feb 15, 2032 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0644
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.