Method for facilitating an automated isolation of a biopolymer using magnetic particles

US8729252B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Ralf Himmelreich · Langenfeld, DE
• Sabine Werner · Mülheim an der Ruhr, DE

Key dates

• Filing date: Aug 16, 2004
• Grant date: May 20, 2014
• Expiry date: Mar 25, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1013
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for isolating polynucleotides, such as DNA, RNA and hybrids thereof from an aqueous solution containing polynucleotides by reversibly binding the polynucleotides to silica-coated magnetic particles in the presence of a salt and non-ionic hydratable additive is disclosed. The salt and non-ionic hydratable additive concentrations are adjusted to levels that result in adhesion of the nucleic acid to the particles without degradation or precipitation of the nucleic acid.