Detection system for droplet-based assays

US8730479B2 · kind B2 · utility

Assignee

• Bio-Rad Laboratories, Inc. · US

Inventors

• Kevin Ness · Pleasanton, US
• Mark Alan Arbore · Palo Alto, US
• Jerry E. Hurst, Jr. · San Jose, US
• David L. Klein · Palo Alto, US
• Donald A. Masquelier · Tracy, US

Key dates

• Filing date: Dec 30, 2011
• Grant date: May 20, 2014
• Expiry date: Dec 30, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2201/0696
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

System, including methods and apparatus, for light detection and signal processing for droplet-based assays. An exemplary system provides a method of detection for droplets. An examination region of a channel may be illuminated with first pulses of light interleaved with second pulses of light as droplets pass through the examination region, the first pulses being spectrally distinct from the second pulses. Data may be collected representing light detected during illumination of the examination region with the first pulses and the second pulses. Each droplet may be illuminated with a beam of light that is narrower than a diameter of the droplets.