Cell extract for cell-free protein synthesis and process for producing the same
US8734856B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 31, 2003 |
| Grant date | May 27, 2014 |
| Priority date | — |
| Expiry date | Sep 11, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for preparing cell extract for high-performance cell-free protein synthesis reactions, comprising the elimination of low molecular weight substances that have protein synthesis inhibitory activity, from cell extracts that have cell-free protein synthesis activity, by such methods as dialysis, gel filtration and ultrafiltration. Also provided is a ready-made cell-extract for cell-free protein synthesis employing this method. Furthermore, the formation of insoluble matter can be reduced by performing the process for eliminating the low molecular weight substances that inhibit protein synthesis in the co-presence of ATP, GTP or amino acids, so as to stabilize the cell extract.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.