Lyase enzymes, nucleic acids encoding them and methods for making and using them

US8735107B2 · kind B2 · utility

Assignee

• Verenium Corporation · US

Inventors

• David Weiner · Echt, NL
• Alexander Varvak · San Diego, US
• Toby Richardson · San Diego, US
• Mircea Podar · San Diego, US
• Ellen Burke · San Diego, US
• Shaun Healey · Carlsbad, US

Key dates

• Filing date: Nov 2, 2011
• Grant date: May 27, 2014
• Expiry date: Dec 15, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y403/01023
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.X═NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R═H or alkyl.