Multiplex screening for lysosomal storage disorders (LSDS)

US8735173B2 · kind B2 · utility

Assignee

• Women's and Children's Hospital · AU

Inventors

• Peter John Meikle · Melbourne, AU
• John Joseph Hopwood · Adelaide, AU
• Douglas Alexander Brooks · Melbourne, AU
• Caroline Dean · McLaren Vale, AU

Key dates

• Filing date: Mar 28, 2012
• Grant date: May 27, 2014
• Expiry date: Mar 28, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6893
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A novel protein profiling method of testing for Lysosomal Storage Diseases (“LSD”) using discovered normalized lysosomal fingerprint patterns. The fingerprint patterns reveal the health of lysosomal organelles, specific LSD, and clinical severity. Multiplexing bead technology for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Compounds, reagents, and methods for identifying and quantifying multiple target enzymes and proteins.