Small molecule-dependent split aptamer ligation
US8735367B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 27, 2012 |
| Grant date | May 27, 2014 |
| Priority date | — |
| Expiry date | Jun 27, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2400/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.