Fluorinated rhodamines as photostable fluorescent dyes for labelling and imaging techniques

US8735444B2 · kind B2 · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Stefan W. Hell · Oebisfelde, DE
• Vladimir N. Belov · Oettingen, DE
• Gyuzel Mitronova · Oettingen, DE
• Mariano Bossi · Olavarría, AR
• Gael Moneron · Paris, FR
• Christian Wurm · Oettingen, DE
• Stefan Jakobs · Oettingen, DE
• Christian Eggeling · Oettingen, DE
• Jakob Bierwagen · Oettingen, DE
• Lars Meyer · Oettingen, DE

Key dates

• Filing date: Jun 26, 2009
• Grant date: May 27, 2014
• Expiry date: Jun 26, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/582
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.