Pentamethine cyanine fluorescent dye with N-substituting at β-position of conjugated chain
US8735601B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 6, 2010 |
| Grant date | May 27, 2014 |
| Priority date | — |
| Expiry date | Dec 30, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6439
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A pentamethine cyanine fluorescent dye with N-substituting at β-position of conjugated chain is disclosed. The dye reacts with biosubstrate including protein, liposome and DNA, and shows excellent selectivity. It has good living cell membrane permeability and shows good selectivity in living cell staining In addition, the dye has good stability and low background fluorescence, and can be used together with other fluorescent dyes having consistent wavelengths for single-channel excitation and multi-channel detection. The dye can be used in the fields of protein labeling and detecting, fluorescence immunoassay and living cell selective imaging and the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.