Quantitative nuclease protection assay (QNPA) and sequencing (QNPS) improvements

US8741564B2 · kind B2 · utility

Assignee

• HTG Molecular Diagnostics, Inc. · US

Inventors

• Bruce E. Seligmann · Tucson, US
• Debrah Thompson · Tucson, US
• Tom Vasicek · Minneapolis, US
• Debra A. Gordon · Tucson, US

Key dates

• Filing date: Apr 26, 2012
• Grant date: Jun 3, 2014
• Expiry date: Apr 26, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5′-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.