Method for producing glucuronic acid conjugate using Saccharomyces cerevisiae
US8741602B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 14, 2011 |
| Grant date | Jun 3, 2014 |
| Priority date | — |
| Expiry date | Feb 14, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/44
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a new method for producing a glucuronide, having excellent productivity and being replaceable with a method using Saccharomyces pombe, and to provide a new means used in this production method. Disclosed are: a transformed Saccharomyces cerevisiae wherein a gene coding for a UDP-glucose dehydrogenase and a gene coding for a UDP-glucose transferase are inserted in a manner such that said genes can be expressed; a transformed Saccharomyces cerevisiae wherein a gene coding for a cytochrome P450 gene is further inserted in a manner such that said gene can be expressed; and a method for producing a glucaronide that includes culturing transformed Saccharomyces cerevisiae in the presence of glucose and a substance to be conjugated, generating the glucuronide of the aforementioned substance to be conjugated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.