Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences

US8748100B2 · kind B2 · utility

Assignee

• The Chinese University of Hong Kong · CN

Inventors

• Yuk-Ming Dennis Lo · Kowloon, CN
• Yu-Kwan Tong · Tuen Mun, CN
• Wai Kwun Rossa Chiu · Hong Kong, CN
• Chunming Ding · Hong Kong, CN

Key dates

• Filing date: Aug 30, 2007
• Grant date: Jun 10, 2014
• Expiry date: Feb 12, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.