Method and apparatus for calibrating a flow cytometer
US8748183B2 · kind B2 · utility
34Cited by
514References
21Claims
0Family size
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Key dates
| Filing date | Feb 7, 2013 |
| Grant date | Jun 10, 2014 |
| Priority date | — |
| Expiry date | Feb 7, 2033 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/2575
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of continuously verifying proper sort calibration in a droplet sorting flow cytometer by selecting a fraction of droplets estimated to have substantially zero probability of containing a particle; applying one charge of a set of charges to the selected droplets in order to form a test stream out of the selected droplets; illuminating the droplets in the test stream; and detecting any light emitted or scattered by any particles in the selected droplets.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.