Activating extraction of demineralized bone matrix

US8753660B2 · kind B2 · utility

Assignees

• The Regents of the University of California · US
• DEPARTMENT OF VETERANS AFFAIRS · US

Inventors

• Keyvan Behnam · Simi Valley, US
• Elsa J. Brochmann-Murray · Santa Clarita, US
• Samuel S. Murray · Santa Clarita, US

Key dates

• Filing date: Nov 29, 2005
• Grant date: Jun 17, 2014
• Expiry date: Mar 13, 2029

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61P43/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Demineralized bone matric (DBM) and native bone morphogenetic protein (nBMP) are complex mixtures of non-collagenous bone proteins. These mixtures contain many of the BMPs that are available as recombinant molecules. Information regarding the presence in these materials of molecules that may affect the availability and activity of the BMPs is very limited. A chemical extraction of DBM, such as using alkali-urea produces a water soluble extractate which inhibits the osteogenic activity of DBM. Noggin, an extracellular BMP ligand antagonist is found in the water soluble extractate from DBM. Differential chemical extraction is a useful means of removing non-osteogenic, or osteogenic inhibitory molecules from DBM and nBMP.