Charge perturbation detection method for DNA and other molecules
US8753812B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 29, 2012 |
| Grant date | Jun 17, 2014 |
| Priority date | — |
| Expiry date | Mar 29, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/3276
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time using a mix of all dNTPs with subsequent analysis of the resulting signal. This technique may be adapted to other reaction determinations, such as enzymatic reactions, other electrode configurations, and other amplifying circuits.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.