Methods of modifying nucleic acids in host cells

US8753846B2 · kind B2 · utility

Assignee

• The University of Nottingham · GB

Inventors

• John Heap · Nottingham, GB
• Nigel P. Minton · Salisbury, GB

Key dates

• Filing date: Feb 13, 2009
• Grant date: Jun 17, 2014
• Expiry date: Jul 25, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/902
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of double crossover homologous recombination in a host cell comprising: a first homologous recombination event between a donor DNA molecule comprising a first element of a selectable allele and an acceptor DNA molecule comprising a second element of the selectable allele in the host cell, thereby to form a product of the first homologous recombination event in the host cell; and a second homologous recombination event within the product of the first homologous recombination event, thereby to form a product of the second homologous recombination event in the host cell which confers a selectable phenotype on the host cell, wherein the selectable phenotype arises following and in dependency on the formation of a selectable allele from the first and second elements of the selectable allele.