Compositions comprising homogeneously glycosylated erythropoietin

US8754192B2 · kind B2 · utility

Assignee

• Sloan Kettering Institute for Cancer Research · US

Inventors

• Samuel J. Danishefsky · New Haven, US
• J. David Warren · New York, US
• Jiehao Chen · Westfield, US
• Bin Wu · Thousand Oaks, US
• Gong Chen · Xiamen, CN
• Qian Wan · Quincy, US
• Zhongping Tan · New York, US
• Cindy Kan · New York, US
• Yu Yuan · Sichuan, CN
• Zihao Hua · Andover, US
• Krishnakumar Ranganathan · Oak Park, US
• John David Trzupek · Medford, US

Key dates

• Filing date: Apr 11, 2007
• Grant date: Jun 17, 2014
• Expiry date: Jun 11, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/005
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides isolated homogeneous polyfunctionalized proteins (e.g., erythropoietin), isolated glycopeptides, and a method for preparing polyfunctionalized peptides and/or proteins via cysteine-free native chemical ligation. In certain embodiments, the invention provides an isolated homogeneous polyfunctionalized protein having the structure (I). In certain other embodiments, the invention provides an isolated glycopeptide having Formula (II). In certain other embodiments, the inventive method is a method for preparing a polyfunctionalized peptide comprising a peptidic backbone made up of four or more amino acids, wherein two or more non-adjacent amino acids are independently substituted with a moiety having the structure (III) -LH. wherein A and L1 are as defined herein.