Methods for increasing recovery of an ADAMTS activity from a cell culture supernatant
US8759026B2 · kind B2 · utility
10Cited by
2References
32Claims
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Key dates
| Filing date | Sep 19, 2012 |
| Grant date | Jun 24, 2014 |
| Priority date | — |
| Expiry date | Sep 19, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.