Amplification methods to minimise sequence specific bias
US8759037B2 · kind B2 · utility
3Cited by
5References
20Claims
0Family size
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Key dates
| Filing date | Feb 23, 2011 |
| Grant date | Jun 24, 2014 |
| Priority date | — |
| Expiry date | Feb 23, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for amplifying nucleic acids are provided. The methods can be used to minimize sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimizing GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilizing flow cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.