Thrombin activator compositions and methods of making and using the same

US8759066B2 · kind B2 · utility

Assignee

• ZYMOGENETICS INC. · US

Inventors

• Paul D. Bishop · Fall City, US
• Tracey A. Pownder · Bellevue, US
• Paul O. Sheppard · Granite Falls, US
• Christopher J. Stenland · Stanwood, US

Key dates

• Filing date: Apr 7, 2009
• Grant date: Jun 24, 2014
• Expiry date: Jan 20, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/50
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are compositions for activating thrombin precursors to thrombin. The compositions provided include polypeptide compositions wherein the pre-pro-sequence comprises a thrombin cleavage site. The compositions provided also include polynucleotides encoding said polypeptides and recombinant systems for expressing said polypeptides. This disclosure also relates to methods for producing said compositions, recovering said compositions, activating said compositions purifying said compositions and producing active thrombin molecules using the active form of said compositions.