Apparatus for selective excitation of microparticles
US8759077B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 2007 |
| Grant date | Jun 24, 2014 |
| Priority date | — |
| Expiry date | Dec 9, 2030 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L7/52
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.