Methods and means for exact replacement of target DNA in eukaryotic organisms

US8765448B2 · kind B2 · utility

Assignee

• Bayer CropScience N.V. · BE

Inventors

• Anne Rolland · Val-de-Reuil, FR
• Manuel Dubald · Raleigh, US
• Michiel Van Lookeren Campagne · Chapel Hill, US
• Rene Ruiter · Melle, BE

Key dates

• Filing date: Jun 3, 2008
• Grant date: Jul 1, 2014
• Expiry date: Jun 29, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8213
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and means are provided for the exact exchange in eukaryotic cells, such as plant cells, of a target DNA sequence for a DNA sequence of interest through homologous recombination, whereby the selectable or screenable marker used during the homologous recombination phase for temporal selection of the gene replacement events can subsequently be removed without leaving a foot-print employing a method for the removal of a selected DNA flanked by two nucleotide sequences in direct repeats.