Method for producing monatin using an L-amino acid aminotransferase
US8771997B2 · kind B2 · utility
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7References
16Claims
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Key dates
| Filing date | Oct 12, 2011 |
| Grant date | Jul 8, 2014 |
| Priority date | — |
| Expiry date | Feb 16, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P17/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.