Method for quantitatively determining a number of analytes
US8778612B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 7, 2003 |
| Grant date | Jul 15, 2014 |
| Priority date | — |
| Expiry date | Aug 31, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/9123
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for the quantitative assay of n different analytes, where n is at least 2, is provided. The method consists of the following steps: (a) At least one labeled detector-binding partner is added to a test sample containing the analytes. This leads to the formation of detector-analyte complexes, each of which consists of one analyte molecule and one detector molecule. The number of detector binding partners x equals n−1. Each detector-binding partner binds to at least one analyte. At least one of the detector-binding partners can bind to at least two analytes. (b) The detector-analyte complexes formed in step (a) bind to capture-binding partners and form detector-analyte-capture complexes. The number of capture-binding partners y equals the number n of the analytes. Each capture-binding partner is specific for at least one detector-analyte complex. (c) The time-resolved formation of the detector-analyte-capture complexes is measured.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.