Mono charging system for selectively introducing non-native amino acids into proteins using an in vitro protein synthesis system

US8778631B2 · kind B2 · utility

Assignee

• Sutro Biopharma, Inc. · US

Inventors

• Alexei M. Voloshin · South San Francisco, US
• James F. Zawada · South San Francisco, US
• Daniel Solomon Gold · Novato, US
• Christopher J. Murray · Philadelphia, US
• James Rozzelle · San Francisco, US
• Nathan Uter · South San Francisco, US
• Gang Yin · South San Francisco, US

Key dates

• Filing date: Jan 12, 2010
• Grant date: Jul 15, 2014
• Expiry date: Apr 13, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.