Optical based delivery of exogenous molecules to cells

US8778682B2 · kind B2 · utility

Assignee

• General Electric Company · US

Inventors

• Vasile Bogdan Neculaes · Niskayuna, US
• Kenneth Roger Conway · Clifton Park, US
• Allen Lawrence Garner · Stratford, US
• Evelina Roxana Loghin · Rexford, US
• Siavash Yazdanfar · Niskayuna, US
• Dmitry Vladimirovich Dylov · Princeton, US
• Brian Michael Davis · Albany, US
• Chulmin Joo · Seojong-myeon, KR

Key dates

• Filing date: Aug 30, 2011
• Grant date: Jul 15, 2014
• Expiry date: Oct 16, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/87
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method of delivering exogenous molecules, comprising: providing a plurality of cells having a cell membrane; adding a plurality of exogenous molecules to the cells; exposing the cells to a defocused infrared (IR) light to permeabilize the cell membrane of the cells; and delivering the exogenous molecules to the cells through the permeablized cell membrane, wherein an intensity of the IR light at the optical focus is at least greater than or equal to an order of 104 W/cm2.