High-throughput methods for determining pharmacological levels of plasma D-serine

US8778688B2 · kind B2 · utility

Assignee

• THE JOHNS HOPKINS UNIVERSITY · US

Inventors

• Barbara Slusher · Kingsville, US
• Jesse Alt · Essex, US
• Camilo Rojas · Baltimore, US
• Takashi Tsukamoto · Ellicott City, US

Key dates

• Filing date: Nov 8, 2012
• Grant date: Jul 15, 2014
• Expiry date: Nov 8, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Described herein are high-throughput methods of monitoring D-serine levels in plasma. The assay involves the use of strong cation solid phase extraction (SPE) to isolate D-serine from plasma, followed by quantitation of D-serine using the D-amino acid oxidase- (DAAO-) catalyzed reaction. Also described are methods of screening for compounds that act as DAAO inhibitors.