Continuous biomolecule separation in a nanofilter
US8783466B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 13, 2011 |
| Grant date | Jul 22, 2014 |
| Priority date | — |
| Expiry date | Feb 7, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/0288
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
This invention provides a method and an apparatus for quickly continuously fractionating biomolecules, such as DNAs, proteins and carbohydrates by taking advantage of differential bidirectional transport of biomolecules with varying physico-chemical characteristics, for example size, charge, hydrophobicity, or combinations thereof, through periodic arrays of microfabricated nanofilters. The passage of biomolecules through the nanofilter is a function of both steric and electrostatic interactions between charged macromolecules and charged nanofilter walls, Continuous-flow separation through the devices of this invention are applicable for molecules varying in terms of any molecular properties (e.g., size, charge density or hydrophobicity) that can lead to differential transport across the nanofilters.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.