Method for quantitative measurements of HDL-C and LDL-C
US8785146B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | Oct 6, 2008 |
| Grant date | Jul 22, 2014 |
| Priority date | — |
| Expiry date | Feb 18, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/60
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for direct measurement of high-density lipoprotein cholesterol. (HDL-C) or low-density lipoprotein cholesterol (LDL-C) based on a modified chemical precipitation method, wherein the improvements were made by optimizing the concentrations and proportions of potassium polyvinyl sulfate (PVSK) and polyethylene glycol methyl ether (PEGME) in such a way that the enzymatic reactions are completed before precipitation formations. The method in combination with certain surfactants showed good correlations with other direct methods used for HDL-C or LDL-C determinations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.