L-threonine analysis method and L-threonine dehydrogenase
US8785147B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 4, 2012 |
| Grant date | Jul 22, 2014 |
| Priority date | — |
| Expiry date | Sep 4, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for analyzing L-threonine contained in an specimen, which includes the steps of mixing a sample containing the specimen with an L-threonine dehydrogenase derived from Cupriavidus necator and a coenzyme NAD+ and analyzing the amount of NADH or 2-amino-3-oxobutyric acid after a predetermined period; an L-threonine dehydrogenase derived from Cupriavidus necator, which is a novel L-threonine dehydrogenase (TDH; EC 1.1.1.103) and can be utilized in the above-mentioned analysis method; a method for preparing a gene or the like to be used in the preparation of the enzyme, or a method for preparing the enzyme; an L-threonine analysis kit which includes (A) the L-threonine dehydrogenase and (B) a coenzyme NAD+; an enzyme preparation for use in the analysis of L-threonine, which includes the L-threonine dehydrogenase contained in a buffer solution; and an enzyme sensor utilizing the L-threonine dehydrogenase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.