Patent · US Active

Cutinase-producing genetically engineered microorganism and use thereof

US8785169B2 · kind B2 · utility

1Cited by

0References

7Claims

0Family size

Assignee

Jiangnan University · CN

Inventors

Jian Chen · Wuxi, CN
Jing Wu · New York, US
Dan Wu · Nanhu, CN
Lei Wang · Beijing, CN

Key dates

Filing date	Feb 28, 2011
Grant date	Jul 22, 2014
Priority date	—
Expiry date	Oct 22, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y301/01074
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to the field of bioengineering and discloses a cutinase-producing genetically engineered microorganism and use thereof. Recombinant plasmid Tfu_0883-hlyAs/pET20b(+) was constructed and transformed into E. coli BL21(DE3) to obtain recombinant E. coli strain Tfu_0883-hlyAs/pET20b(+)/E. coli BL21(DE3). Specific growth rate was maintained at a certain value using fed-batch fermentation mode. After fermenting 30-34 hours, the enzyme activity in the supernatant reached 700-750 U/mL. The present invention uses glycerol as the main raw material and employs semi-synthetic medium, has the advantages of good stability and ease of control, and is suitable for large-scale production.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.