Method of preparing carrier to separate nucleic acids, carrier and micro channel to separate nucleic acids, and method and apparatus for separating nucleic acids
US8785348B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 29, 2009 |
| Grant date | Jul 22, 2014 |
| Priority date | — |
| Expiry date | Feb 25, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to a method and apparatus for separating nucleic acids. A carrier may include a porous microbead having cation-exchangeable groups attached to the surface of the porous microbead. Capturing chains modified with positively charged functional groups and having a base sequence complementary to a target nucleic acid chain sequence are immobilized on to the surface of the porous microbead. In various embodiments, capturing chains are immobilized on to the surface of the porous microbead through an ion exchange bond or a covalent bond with the cation-exchangeable groups of the porous microbead. In some cases, the porous microbead has a number of through pores adapted to permit a solution to pass rapidly through the through pores and a number of diffusive pores adapted to permit a solute of the solution to diffuse into the diffusive pores. A solution having a target nucleic acid chain corresponding to the target nucleic acid chain sequence of the capturing chains may be passed through a channel that is packed with porous microbeads so as to result in hybridization of the capturing chain and the target nucleic acid chain. The porous microbeads may be structur…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.