SlpA as a tool for recombinant protein and enzyme technology

US8790659B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Christian Scholz · Penzberg, DE
• Elke Faatz · Huglfing, DE
• Urban Schmitt · Oberhausen, DE
• Peter Schaarschmidt · Ulm, DE

Key dates

• Filing date: Aug 8, 2012
• Grant date: Jul 29, 2014
• Expiry date: Aug 8, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.