Fibrous assemblies for antibody presentation, and multiplexed antigenic analysis using same
US8791239B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 2009 |
| Grant date | Jul 29, 2014 |
| Priority date | — |
| Expiry date | Dec 7, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC08L5/08
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Biofunctionalized fibers including a fiber platform and a histidine-tagged protein and, optionally, an antibody. Chitosan is a fiber useful as the fiber platform. The fiber platform may be treated with nickel or may be directly linked to the histidine-tagged protein e.g., histidine-tagged streptococcal IgG-binding protein, protein G, protein G3T, GFP or RFP. The resulting biofunctionalized fibers can be assembled into protein fiber assemblies by a variety of biofabrication methods. The fiber assemblies, e.g., in the form of woven fabrics, are useful for (i) antigen capture; (ii) immunoanalysis, and/or (iii) multiplexed analysis. In one fabrication, each fiber of a fiber assembly presents a specific antibody, and mixing and matching of fibers, for example by weaving of fabrics from various antibody-presenting fibers, allows for multiple antigens to be captured simultaneously for multiplexed analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.