Enzymatic substrates for multiple detection systems
US8791246B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 3, 2012 |
| Grant date | Jul 29, 2014 |
| Priority date | — |
| Expiry date | Apr 3, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/15
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An inventive substrate is provided which includes a substrate compound of formula A-B1-B2-B3-B4: wherein A is a sugar moiety; B1 is a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; B2 is a linker moiety with a free reactive amino group so as to be available for reaction with carboxylic acids or detectable tags; B3 contains a permanently charged element such as a quaternary ammonium group so as to increase sensitivity for mass spectrometry analysis; and B4 of various carbon length conferring specificity amongst individual substrates in detection methods. Also provided is a molecule of the formula B1-B2-B3-B4, with similar structural characteristics to an enzymatic product produced by the action of a target enzyme on an inventive substrate. Further provided are methods for using inventive substrates for detecting enzymatic activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.