Primer-extension based method for the generation of siRNA/miRNA expression vectors
US8795988B2 · kind B2 · utility
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Key dates
| Filing date | Aug 15, 2008 |
| Grant date | Aug 5, 2014 |
| Priority date | — |
| Expiry date | Jun 25, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Functional shRNA is produced from an expression vector prepared by selecting a two primer design in which the primers are less than about 50 nucleotides in length, annealing and extending the primers using primer extension, digesting the primer extension product and inserting the digestion product into a suitable vector. When the shRNA vectors are inserted into a cell, shRNA transcribed from the vectors modulates gene activity within the cell.
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