Method for protein isolation in anoxic conditions
US8802395B2 · kind B2 · utility
1Cited by
10References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 9, 2012 |
| Grant date | Aug 12, 2014 |
| Priority date | — |
| Expiry date | May 9, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for the isolation of proteins that comprise disulfide bonds in their native conformation. Essentially, a method of the present invention makes use of reducing agents such as β-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.