Engineered landing pads for gene targeting in plants

US8802921B2 · kind B2 · utility

Assignee

• DOW AGROSCIENCES LLC · US

Inventors

• William M. Ainley · Carmel, US
• Ryan C. Blue · Fishers, US
• Michael G. Murray · Ballina, IE
• David R. Corbin · Chesterfield, US
• Rebecca Ruth Miles · Pendleton, US
• Steven R. Webb · Westfield, US

Key dates

• Filing date: Jan 21, 2011
• Grant date: Aug 12, 2014
• Expiry date: Nov 10, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8241
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.