Method for determining the activity of a proteolytic coagulation factor in a sample

US8809007B2 · kind B2 · utility

Assignee

• Siemens Healthcare Diagnostics Products GmbH · DE

Inventors

• Gerlinde Christ · Marburg, DE
• Andreas Kappel · Höhenkirchen-Siegertsbrunn, DE

Key dates

• Filing date: Aug 5, 2013
• Grant date: Aug 19, 2014
• Expiry date: Aug 5, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/96433
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for determining the activity of a proteolytic coagulation factor in a sample may include (a) providing and incubating a reaction mixture comprising (i) the sample, (ii) an agent for direct or indirect activation of the proteolytic coagulation factor in the sample, (iii) a cleavable substrate which has at least one cleavage site for the activated coagulation factor, (iv) a solid phase to which the cleavable substrate is bound or becomes bound during the incubation; (b) separating off the solid phase; and (c) determining the amount of solid-phase-bound, uncleaved substrate, wherein the determined amount of solid-phase-bound, uncleaved substrate indicates a quantitative measure of the activity of the proteolytic coagulation factor in the sample.