Method for culturing mycoplasma contamination-free cells and method for removing mycoplasma contamination of cells
US8809034B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 26, 2013 |
| Grant date | Aug 19, 2014 |
| Priority date | — |
| Expiry date | Nov 26, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/76
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method for culturing cells, which prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using a fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, provided is a method for removing mycoplasma contamination of the cells, including a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.