Plasmid vector

US8809048B2 · kind B2 · utility

Assignee

• INDEPENDENT ADMINISTRATIVE INSTITUTION, JAPAN AGENCY FOR MARINE-EARTH SCIENCE AND TECHNOLOGY · JP

Inventors

• Yukari Ohta · Yokosuka, JP
• Yuji Hatada · Yokohama, JP
• Kozue Mori · Yokosuka, JP
• Nobuyuki Nakamura · Yokosuka, JP

Key dates

• Filing date: Mar 18, 2011
• Grant date: Aug 19, 2014
• Expiry date: Mar 18, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/74
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The object is to provide a technique for modifying a bacterium belonging to the genus Kocuria through genetic engineering for industrially effectively utilizing the bacterium belonging to the genus Kocuria. The above object can be achieved by providing a cyclic plasmid, which has a replication region comprising the base sequence of a DNA-binding protein-like protein gene, the base sequence of a replicase-like protein gene, and the base sequence represented by SEQ ID NO:45, and is autonomously replicable in bacteria. As an example of the aforesaid plasmid, a plasmid containing a base sequence represented by SEQ ID NO:3 or 4 which originates in Kocuria sp. MBE131 strain (FERM P-21885) can be cited.