Fluorescence-based assay for the rapid detection and quantification of deoxyribonucleoside triphosphates
US8815509B2 · kind B2 · utility
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Assignee
Inventors
Key dates
| Filing date | Jun 14, 2012 |
| Grant date | Aug 26, 2014 |
| Priority date | — |
| Expiry date | Jun 14, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6428
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The inventors have developed a rapid and sensitive fluorescence-based assay to quantify dNTPs. This assay relies on the principle that incorporation of a limiting dNTP is required for primer-extension and polymerase-mediated 5-3′ exonuclease hydrolysis of a quenched fluorophore-labeled probe resulting in fluorescence. The concentration of limiting dNTPs is directly proportional to the fluorescence generated. This assay has important applications in research that investigates the influence of pathological conditions or pharmacological agents on dNTP biosynthesis and regulation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.