Sulfotransferase assay
US8815529B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 23, 2011 |
| Grant date | Aug 26, 2014 |
| Priority date | — |
| Expiry date | Jan 26, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2533/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Assays and methods for detecting and/or quantifying activity of a test sulfotransferase comprising by releasing a free phosphate from the PAP produced by the sulfotransferase reaction and detecting the free phosphate. The assay can includes gPAPP, a free phosphate detector and an optional control sulfotransferase. The method includes combining a test sulfotransferase with the substrate of the sulfotransferase, PAPS and gPAPP and detecting the free phosphate. The level of free phosphate directly correlates to the activity of the sulfotransferase. The assay and methods can be used to screen agents for their effect upon sulfotransferase activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.